Identification and Behavioral Assays of Alarm Pheromone in the Vetch Aphid Megoura viciae

نویسندگان

چکیده

Aphids are destructive pests, and alarm pheromones play a key role in their chemical ecology. Here, we conducted detailed analysis of terpenoids the vetch aphid, Megoura viciae, its host plant Pisum sativum using gas chromatography/mass spectrometry. Four major components, (-)-?-pinene (49.74%), (E)-?-farnesene (32.64%), (-)-?-pinene (9.42%) ( +)-limonene (5.24%), along with trace amounts +)-sabinene, camphene ?-terpineol) (3.14%) were found aphid. In contrast, few plant, consisting mainly squalene (66.13%) analog 2,3-epoxysqualene (31.59%). Quantitative four terpenes different developmental stages aphid showed that monoterpenes increased increasing stage, while sesquiterpene amount peaked 3rd instar. (-)-?-Pinene was most abundant terpene at all stages. Behavioral assays three-compartment olfactometer revealed repellency single compounds varied concentration-dependent manner, but two mixtures [(-)-?-pinene: (-)-?-pinene: (E)-?-farnesene: = 1:44.4:6.5:2.2 or 1:18.4:1.3:0.8], repellent concentrations tested. Our results suggest active components pheromone M. response. study contributes to understanding ecology aphids may help design new control strategies against this pest.

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ژورنال

عنوان ژورنال: Journal of Chemical Ecology

سال: 2021

ISSN: ['0098-0331', '1573-1561']

DOI: https://doi.org/10.1007/s10886-021-01297-4